The cell culture plate can be divided into a flat bottom and a round bottom (U-shaped and V-shaped) according to the shape of the bottom; the number of holes in the culture hole is 6, 12, 24, 48, 96, 384, 1536, etc.; Terasaki plates and common cell culture plates.
Differences and choices between flat and round bottom (U and V) plates
Different shapes of culture plates have different uses. The cultured cells are usually flat-bottomed, which is convenient for microscopic observation, clear bottom area, and relatively uniform cell culture surface height. Therefore, when performing experiments such as MTT, whether it is adherent or suspended cells, a flat bottom plate is generally used. The absorbance must be measured using a flat-bottomed plate. Pay special attention to the material and mark "Tissue Culture (TC) Treated" for cell maintenance.
U-shaped or V-shaped plates are generally used only for certain special requirements. For example, in immunology, when two different lymphocytes are mixed and cultured, it is necessary to contact each other with stimulation. In this case, a U-shaped plate is generally used because the cells are concentrated in a small range due to the action of gravity. Round bottom culture plates are also used for isotope incorporation experiments, and cell cultures such as "mixed lymphocyte culture" are required to collect cells. V-shaped plates are commonly used for cell killing and immunological blood coagulation experiments. Cell killing This experiment can also be replaced with a U-shaped plate (after centrifugation, low-speed centrifugation).