Cell culture plate shape requirements

- Jul 04, 2019-

According to the shape of the bottom, it can be divided into flat bottom and round bottom (U-shaped and V-cell culture plate type)

What type of cells are available at the bottom, but when the number of cells is small, such as cloning, a 96-well flat bottom plate is used. In addition, when performing experiments such as MTT, flat bottom plates are generally used for adhering and suspending cells. As for U or V-type boards, they are generally used only for certain special requirements. For example, in immunology, when two different lymphocyte complexes are cultured, they need to be in contact with each other to stimulate. Therefore, U-shaped plates are generally required, because cells are concentrated in a small range due to the action of gravity. V-plates are less useful. They are commonly used in cell killing experiments. In order to make the target cells in close contact, V-plates are often used, but this test can also be replaced by U-plates (after centrifugation, low-speed centrifugation). If you raise cells, you usually use a flat bottom. In addition, you should pay special attention to the material. It seems that you have not heard of the cells at the bottom. The round bottom is usually used for analysis, chemical reaction, or for storing samples. Because the round bottom is better to suck the liquid clean, if it is flat, it will not suck. However, if you want to measure the absorbance, you must buy a flat bottom. Most cell cultures use flat-bottom culture plates, which are convenient for microscopic observation, clear bottom area, relatively uniform cell culture liquid level, and easy for MTT detection.

Round bottom culture plates are mainly used for isotope incorporation experiments, and cell culture is required to collect cells.