Generally, it is subjected to four steps of soaking, brushing, pickling, and washing.
1. Soaking: New or used glassware should be soaked in water to soften and dissolve the deposits. New glassware must be simply brushed with tap water before use, and then soaked overnight with 5% hydrochloric acid; used glassware is often accompanied by a large amount of protein and grease, which is not easy to brush off after drying, so it should be immersed in clean water immediately after use. .
2. Brushing: Place the soaked glassware in the detergent water and brush it repeatedly with a soft brush. Do not leave dead ends and prevent damage to the surface of the vessel. Wash and dry the brushed glassware for pickling.
3. Pickling: Pickling the above-mentioned utensils into the cleaning solution, also known as acid, removes possible residual substances on the surface of the vessel by strong oxidation of the acid. The pickling should be no less than six hours, usually overnight or longer. Be careful when handling the utensils.
4. Flushing: All the utensils after brushing and pickling must be washed thoroughly with water. Whether the utensils are rinsed clean after pickling, directly affects the success or failure of cell culture. Manually wash the dishes after pickling, each piece must be repeatedly "filled in water - empty" more than 15 times, and finally dip 2-3 times with steamed water, dried or dried and packaged for use.