Use of cytometer
How to use an automatic cell counter
Recommended Visit: Factors and Solutions Affecting the Accuracy of Cell Counting How to Choose the Function of a Cell Counter Multifunctional Blood Cell Counter Living Cell Counting Introduction to the safety instructions and cleaning and maintenance of an automatic cell counter
The cell counter is a microcomputer-controlled counting instrument that integrates counting, statistics, and display. The count is accurate and reliable, consistent with the blood test results of clinical tests, and preliminary hints to distinguish bacterial or viral infections. The cell counter is easy to use, and it is especially suitable for the classification and counting of cells in clinical tests in small and medium hospitals.
Cell counter operation steps
1. Press the Power button to turn on the instrument. The opening interface is displayed.
2. The automatic cell counter is preset to Cell mode. If it is another mode, please change to Cells mode.
3. Add 10 μL of sample to 10 μL trypan blue dye, pipette up and down, and mix gently.
4. Add 10 μL of the sample mixture to the counting cell port on the side of the cell counting plate. The two counting cells of the counting board are labeled "A" and "B", for easy tracking of your samples. You can use one counting cell for cell counting, or you can use both.
. Insert the cell counting board. First insert the A-side sample into the cell counting board interface. You must ensure that the A-side sample is completely inserted into the instrument. If the cell counter is inserted correctly, you will hear a click. Each counting pool counts separately.
6. Press the CountCells or NextSample button.
7. Press the Zoom button to adjust the image. Navigate in the grid by clicking on the target area.
8. When viewing cells in zoom mode, you can use the focus knob to adjust the image. It is not necessary to use the focus knob again after counting the first sample. You can lock the focus knob if multiple samples are of similar size. You can also unlock the focus knob later to adjust the image. Adjust the image so that the center of live cells (light cells) is bright, the edges are dark, and the entire cell area of dead cells (dead cells) is uniformly blue with no central bright spots for data analysis.
9. If the image looks good, press CountCells.
10. It takes about 30 seconds for the instrument to finish counting a sample. The screen will display the number of live cells, the number of dead cells, the total number of cells, and the percentage of survival. Record the cell count results or insert a USB drive and save the data.
11. After counting is complete, press the Details button to view the count of each cell. Cell conditions are shown by blue, red or black circles. Blue means cells are counted as living cells,