Why must immediately after sterilization medium preparation? How to check the sterilization after the culture medium is sterile?
Culture medium preparation, if is used in cell culture, due to the various cells in the air and all kinds of ingredients is very much, if not first sterilization, in cultivating brought interference is due to the bacteria in the background, using the experiment failed.
To check out the effect of culture medium method is very simple, put to medium temperature experiment box for culture (same time and training experiment, usually 24 hours) after the sterilization medium colony can judge. Sometimes in order to use the training more scientific, was in a bacterial culture, in addition to the strains of the paint on the need to develop, at the same time plus a medium composition (blank) simultaneously with the condition were compared.