1. Use a suitable tip:
To ensure better accuracy and accuracy, it is recommended that the amount of pipetting be within the 35%-100% range of the tip.
2. Installation of the tip:
For most brands of pipettes, especially multi-channel pipettes, it is not easy to install the tip: in order to achieve a good seal, you need to insert the pipette handle into the tip, turn it left and right or shake it back and forth. . Some people will use the pipette to repeatedly hit the tip to tighten, but this operation will cause the tip to deform and affect the accuracy, and serious will damage the pipette, so this operation should be avoided.
3. Tip immersion angle and depth:
The tip immersion angle is controlled within 20 degrees of inclination and is kept vertical; the tip immersion depth is recommended as follows:
Pipette specifications Tip immersion depth
2μL and 10 μL 1 mm
20μL and 100 μL 2-3 mm
200μL and 1000 μL 3-6 mm
5000 μL and 10 mL 6-10 mm
4. Tip rinse:
For normal temperature samples, the tip rinse helps to improve accuracy; however, for high temperature or low temperature samples, the tip rinse reduces the accuracy of the operation, so please pay special attention.
5. Aspirate speed:
The pipetting operation should maintain a smooth, suitable pipetting speed; too fast aspiration rate can easily cause the sample to enter the sleeve, causing damage to the piston and seal and cross-contamination of the sample.