The E. coli O157:H7 PCR detection kit is a rapid and accurate kit for the qualitative determination of Enterobacter aeruginosa O157:H7. Based on the principle of PCR combined with enzyme-linked immunosorbent assay for amplified samples, a product with high detection sensitivity was developed. A direct test can be achieved with a bacterial concentration of 103 cfu/ml. During the PCR process, a specific piece of DNA is amplified and detected by hybridization with a probe that labels biotin. After the substrate enzyme is cultured, the results of the sample can be read by visual discrimination or a microplate reader. The kit uses a specific primer of E. coli O157:H7 to specifically bind to the corresponding target site of the E. coli O157:H7 genome in the sample, and achieves the rapid detection of E. coli O157:H7 by PCR reaction. The kit is highly specific and can specifically distinguish E. coli O157:H7 from other bacteria.